ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (2): 327-332.doi: 10.11843/j.issn.0366-6964.2014.02.023

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Expression and Location Analysis of APC in Alpaca Skin

ZHANG Dan-li1, TIAN Xue2,FAN Rui-wen1,YU Xiu-ju1,SONG Yun-fei3,DONG Chang-sheng1*   

  1. (1.College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801, China;2.College of Fisheries, Henan Normal University,Xinxiang 453007, China;3.BGI,Lanzhou 730000, China)
  • Received:2013-10-06 Online:2014-02-23 Published:2014-02-23

Abstract:

To study the function of APC gene in alpaca coat color formation,the expression and localization of APC in different alpaca coat color were investigated.Quantitative real-time PCR (QRT-PCR) and immunohistochemistry were used to detected the mRNA expression level and the location of APC protein in brown and white alpaca skin.The gene expression quantity of APC in brown alpaca skin was 5.042 2 times than white one.The results of immunohischemistry revealed that the APC positive signals were found in hair bulb,hair root and epidermis in alpacas.Moreover,the average protein expression of APC in brown alpaca skin was higher than that in white one based on the average optical density (P<0.05).Based on the results,it indicated that APC may involve in alpaca coat color formation.

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